Dear all,
I am trying to optimize my crystal with additives. Since the yield of my
protein purification is very limited, I am wondering what is the most efficient
way to set up drops with additive to save my protein and not wasting additives?
I am setting up 1 to 1 drops with 0.2 ul additives. But I feel 0.2ul is not
very actuate, even if I use a p2. Would you share your ways to set up drops
with additives? If I want to screen some additives, what additives would you
suggest to try first, especially those from the 96 additive conditions from
Hampton? By the way, just wondering, what kind of p2 pipettor work better? Any
input is greatly appreciated!
Thank you,
Min
