Use robot. You only need 0.1ul*96=9.6ul of protein solution.
On Thu, Mar 3, 2011 at 7:59 PM, m zhang <[email protected]> wrote: > Dear all, > > I am trying to optimize my crystal with additives. Since the yield of my > protein purification is very limited, I am wondering what is the most > efficient way to set up drops with additive to save my protein and not > wasting additives? I am setting up 1 to 1 drops with 0.2 ul additives. But I > feel 0.2ul is not very actuate, even if I use a p2. Would you share your > ways to set up drops with additives? If I want to screen some additives, > what additives would you suggest to try first, especially those from the 96 > additive conditions from Hampton? By the way, just wondering, what kind of > p2 pipettor work better? Any input is greatly appreciated! > > Thank you, > > Min >
