Hi Sadaf, You may want to try to play with the occupancy. I have had a case before where the disulfide bond had double occupancy. In my case it was obvious because I could see the other density, but sometimes you can just see a major one due to disorder.
I hope that helps, Mario Sanches On Sun, Mar 6, 2011 at 1:57 PM, sadaf iqbal <[email protected]> wrote: > Hello everyone, > > Recently, i have solved a protein structure at 1.3 Å resolution. I have > encountered one problem in this structure while refining in COOT. There are > three disulfide bridges in my structure and if i used 2.0 Å resolution > range in refmac then the density around disulfide bridges is ok but if i > used 1.3 Å resolution range in refmac then red density is appeared along > with blue one. Can anybody help me in this regard? I already played by > increasing temperature factor of sulfur atoms but red density is still > there. > > Thanks in advance > > Sadaf Iqbal > PhD Scholar > ICCBS, University of Karachi, Pakistan. > & Visiting Scientist > University of Hamburg, Germany. > > > -- Mario Sanches Postdoctoral Fellow Samuel Lunenfeld Research Institute Mount Sinai Hospital 600 University Ave Toronto - Ontario Canada M5G 1X5 http://ca.linkedin.com/in/mariosanches
