Hi, To start with it would be great if you look in to the secondary structure prediction of the sequence using any of the standard servers like PSIPRED, JPRED etc. Many more available at expasy site http://ca.expasy.org/tools/. Whatever construct you finally choose to make just remember the standard rule that we generally follow is to avoid deleting the alpha helices and beta sheets. You can design your initial primers so as to obtain the complete amplification of these secondary structures from any part within the protein. You can even use the various modules of the following online available server http://scratch.proteomics.ics.uci.edu/ to have an idea of the intrinsically disordered regions in the protein, transmemebrane regions and disulfide bonds that would certainly help you in initiating in the right direction.
Best wishes Gauri On Mon, Mar 7, 2011 at 4:10 AM, Xianhui Wu <[email protected]> wrote: > Dear all, > > Before we try to study the crystal structure of an unknown protein, we > need to determine the sequence that can fold into a compact and stable 3D > domain. What kinds of methods can we choose? > > -- > Best regards, > XH Wu >
