Dear all, Sorry for this off topic question.
We are working on protein/inhibitor complex structure although we can not get our inhibitors in. However, we did find a strange density at the active site, it looks really like GSH, the natural co-enzyme of thiis protein.We tried to use very simple solution to get crystal then exclude the possiblity of buffer moleculors,but that density is aways there. I am wondering how this ligand (if it is GSH) can survive all purification steps and want to indentify it. Are there any methodes to do this work? Let's say to pick up a crystal and do some analysis? Many many thanks!!! Xiaopeng
