Dear Catrine
can you comment on the pH range of your crystallization hits compared to the pH 
of the sample you use for your crystallization trials? The pH can be a major 
issue depending on the nature of the interaction interface.

best regards
Savvas







On 14 Mar 2011, at 13:21, Catrine Berthold Siöberg wrote:

> Dear CCP4bb,
> I am trying to solve the structure of a protein-protein complex. The two 
> proteins are co-expressed and co-purified and I get a homogeneous 1:3 
> complex, which is the predicted composition. I get several hits using 
> commercial crystallisation screens (including the MD ProPlex screen), but all 
> crystals only contain the trimeric, more stable protein of the two.
> 
> I am now considering to crosslink them, but my problem is that I cannot 
> modify them separately to get a specific crosslink, since one of the proteins 
> precipitates by itself.
> I would appreciate all protocols for crosslinking of proteins aimed for 
> crystallisation, or references to structures solved of crosslinked proteins.
> 
> Thank You,
> Catrine
> 
> -- 
> Catrine Berthold Siöberg, PhD
> Stockholm Center for Biomembrane Research
> Department of Biochemistry and Biophysics
> Stockholm University
> S-106 91 Stockholm
> Sweden
> e-mail: catrine.siob...@dbb.su.se
> phone: +468-162451

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