Working with the H+-ATPase AHA2 (C12E8/DDM/Cymal-5 detergent mixed
micelle) we always saw a ring at ~40Å which we attributed to the
micelle, but we have no experimental evidence that it was indeed caused
by detergents.
Heres a animated gif of this phenomenon:
http://www.bioxray.au.dk/~bjopp/pictures/zoom.gif
Also recently I tested some very nice looking and surprisingly hexagonal
xtal-like structures in a drop full of DDM crystals. I got this '6-fold'
pattern:
http://www.bioxray.au.dk/~bjopp/pictures/ddm_diff.png
Again I'm guessing detergent, but I would like to hear any alternative
explanations. :)
hth
-Bjørn
--
Bjørn Panyella Pedersen, PhD
Postdoc
Macromolecular Structure Group
Dept. of Biochemistry and Biophysics
University of California, San Francisco
MC2240, 600 - 16th Street S414
San Francisco, CA 94158-2517
Phone: +1 415-476-3937
E-mail: [email protected]
http://www.msg.ucsf.edu
On 2011-03-19 16:19, <Rain Field> wrote:
Hi All,
I am wondering if the detergent or lipid crystal can have diffraction at low
resolution.
If they can, what does the diffraction pattern looks like? Are there any
literatures describing these?
Many thanks!
