Pius,

Are you sure that you determined the correct cell. Which program did you use? Usually there are much less spots on an image when a crystal has so small unit size dimensions. To me the first crystal looks like protein. Send it to a synchrotron and process the data in XDS. They can process for you.

Maia


On 21/03/2011 4:33 PM, Maia Cherney wrote:
Hi PS

What is the unit cell dimensions in the first crystal? It looks like protein to me.

Maia



 On 21/03/2011 2:03 PM, Pius Padayatti wrote:
Hi all,
We recently observed some diffraction from membrane protein crystallization drops diffraction that look like non-proteinaceous (please see attached files,
from 4 different crystals grown in different conditions).
Rains' question about about lipid and detergent diffraction is so relevant.

This is most likely what lipids and detergent diffraction looks like?
People with similar experience and know what could be these patterns
might be from may have better suggestions and
would like to hear all comments.

first four images are from drops where detergent is DDM and and vapor diffusion
while last image is from a crystal grown in mesophase (with monoolein).

Padayatti PS


On Sat, Mar 19, 2011 at 7:19 PM,<Rain Field> <[email protected]> wrote:
Hi All,
I am wondering if the detergent or lipid crystal can have diffraction at low resolution. If they can, what does the diffraction pattern looks like? Are there any literatures describing these?
Many thanks!


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