The protein purifies nicely there is no problem in that. Just at the last step when it is concentrated it starts precipitating beyond a concentration of 1mg/ml. Already the purification buffers have the detergent.
On Wed, Mar 23, 2011 at 1:44 PM, Kornelius Zeth < [email protected]> wrote: > > 2 M urea and detergents > > On Wed, 23 Mar 2011 13:41:55 -0400 > gauri misra <[email protected]> wrote: > > Hi, > > What are the different methods to prevent protein aggregation while > > concentrating so as to increase the concentration of the protein? > > I have some idea of adding EDTA and charged amino acids like L-Arg and > > L-Glu. > > I would appreciate if the readers share their experiences. > > > > Thanks! > > > > Gauri > > ---------------------------------------------- > Kornelius Zeth > Max Planck Institute for Developmental Biology > Dept. Protein Evolution > Spemannstr. 35 > 72076 Tuebingen, Germany > [email protected] > Tel -49 7071 601 323 > Fax -49 7071 601 349 >
