Try different detergents. Try 10% or more glycerol. Try adding ligands (if present/known). Try varying ionic strength and/or pH. Try giving more specifics so people on the board may be able to help you better.
Bert On 3/23/11 1:51 PM, "gauri misra" <kamga...@gmail.com> wrote: The protein purifies nicely there is no problem in that. Just at the last step when it is concentrated it starts precipitating beyond a concentration of 1mg/ml. Already the purification buffers have the detergent. On Wed, Mar 23, 2011 at 1:44 PM, Kornelius Zeth <kornelius.z...@tuebingen.mpg.de> wrote: 2 M urea and detergents On Wed, 23 Mar 2011 13:41:55 -0400 gauri misra <kamga...@gmail.com> wrote: > Hi, > What are the different methods to prevent protein aggregation while > concentrating so as to increase the concentration of the protein? > I have some idea of adding EDTA and charged amino acids like L-Arg and > L-Glu. > I would appreciate if the readers share their experiences. > > Thanks! > > Gauri ---------------------------------------------- Kornelius Zeth Max Planck Institute for Developmental Biology Dept. Protein Evolution Spemannstr. 35 72076 Tuebingen, Germany kornelius.z...@tuebingen.mpg.de Tel -49 7071 601 323 Fax -49 7071 601 349
