Agitation can cause denaturation of proteins resulting in loss of
activity, precipitation and even cross-beta "amyloid" fibre growth.
Partial unfolding will probably make most proteins more protease sensitive.
Alun.
On 31/03/2011 20:41, gauri misra wrote:
Just an offshoot of the same Question..
I would like to ask whether the same applies for GST-tag digestion
using thrombin..
No agitation gives better results in the above case too...
Any personal experiences....
On Thu, Mar 31, 2011 at 11:29 AM, Klaus Piontek
<[email protected]
<mailto:[email protected]>> wrote:
And not at full moon!
Klaus
Am 31.03.2011 um 16:23 schrieb Xiaopeng Hu:
Our experience is do not shake the tube during TEV cleavage,I
dont know why, but it does help.
xiaopeng
Dr. Klaus Piontek
Albert-Ludwigs-University Freiburg
Institute of Organic Chemistry and Biochemistry, Room 401 H
Albertstrasse 21
D-79104 Freiburg Germany
Phone: ++49-761-203-6036 <tel:%2B%2B49-761-203-6036>
Fax: ++49-761-203-8714 <tel:%2B%2B49-761-203-8714>
Email: [email protected]
<mailto:[email protected]>
Web: http://www.chemie.uni-freiburg.de/orgbio/w3platt/
--
Alun R. Coker
Centre for Amyloidosis and Acute Phase Proteins
Division of Medicine (Royal Free Campus)
University College London
Rowland Hill Street
London
NW32PF
Tel: +44(0)20 7433 2764
Fax: +44(0)20 7433 2776
