if the PCR machine is just to be used for standard sub-cloning (amplifying 
fragments from other plasmids, cloned cDNA etc.), I would go for the cheapest 
one I could find. I guess there are few crystallography projects were the first 
PCR step turned out to be the most difficult. 
For more sophisticated applications there are probably forums where more 
knowledgeable people reside (on PCR that is...) 
Mark 

Quoting "Bernhard Rupp (Hofkristallrat a.D.)":

> Dear All,
>
> I was polled for a  recommendation for a  good PCR instrument,
> but I am not much of a molecular biology person - if someone could
> please help and kindly send some recommendations to
>
> Eric W. Reinheimer <[email protected]>
>
> Best regards, BR
> -----------------------------------------------------------------
> Bernhard Rupp
> 001 (925) 209-7429
> +43 (676) 571-0536
> [email protected]
> [email protected]
> http://www.ruppweb.org/ 
> -----------------------------------------------------------------
> No animals were hurt or killed during the
> production of this email.
> -----------------------------------------------------------------
>

Mark J van Raaij
Laboratorio M-4
Dpto de Estructura de Macromoléculas
Centro Nacional de BiotecnologĂ­a - CSIC
c/Darwin 3, Campus Cantoblanco
28049 Madrid
tel. 91 585 4616
email: [email protected]

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