"I am too poor to afford cheap things" (I am not sure where quote comes from but it's relevant).
The answer depends on whether time is considered expensive or not. Sometimes (e.g. when free labor such as graduate students) time is not an issue. And sometimes it is. Reagent costs are fairly low now. For time, one cannot beat the Robocycler. It's a funny looking design, but it's really fast and quite accurate (since the blocks do not change temperature throughout the process). Artem On Sun, Apr 3, 2011 at 2:42 PM, VAN RAAIJ , MARK JOHAN < [email protected]> wrote: > if the PCR machine is just to be used for standard sub-cloning (amplifying > fragments from other plasmids, cloned cDNA etc.), I would go for the > cheapest one I could find. I guess there are few crystallography projects > were the first PCR step turned out to be the most difficult. > For more sophisticated applications there are probably forums where more > knowledgeable people reside (on PCR that is...) > Mark > > Quoting "Bernhard Rupp (Hofkristallrat a.D.)": > > > Dear All, > > > > I was polled for a recommendation for a good PCR instrument, > > but I am not much of a molecular biology person - if someone could > > please help and kindly send some recommendations to > > > > Eric W. Reinheimer <[email protected]> > > > > Best regards, BR > > ----------------------------------------------------------------- > > Bernhard Rupp > > 001 (925) 209-7429 > > +43 (676) 571-0536 > > [email protected] > > [email protected] > > http://www.ruppweb.org/ > > ----------------------------------------------------------------- > > No animals were hurt or killed during the > > production of this email. > > ----------------------------------------------------------------- > > > > Mark J van Raaij > Laboratorio M-4 > Dpto de Estructura de Macromoléculas > Centro Nacional de Biotecnología - CSIC > c/Darwin 3, Campus Cantoblanco > 28049 Madrid > tel. 91 585 4616 > email: [email protected] >
