Hi, I had set up crystallization with a bicine as buffer and peg 400 as precipitant. I used the detergent DDAO/LDAO as an additive to the crystallization drop (one of the hampton additive screen condition, it says 5% on the vial) I have a clear drop and in the centre there is a shiny precipitate (looks like granules attached to eah other). Then I opened the drop to touch those granules with a needle, but suprisingly its like a skin and the skin surrounded my needle, and then some how I was able to but back the skin in the ppt. orelse the drop is clear if I remove the skin from the drop. I couldnot see the same on the control drop with no protein and just buffer. Does anyone have any experience with such kind of of shiny skins on crystallization drops??
Is it protein? Can I go forward and use it for seeding?? Please suggest with regards Rashmi
