Rashmi,
Yes, you should use the needle for seeding.
The first thing to do is to seed your control drop to make sure that the
needles
are not bicine, as this buffer can give needles at certain pH at high
PEG400
concentrations.
Next (or at the same time) also seed other protein drops that you have
already set up
under similar conditions. I recommend streak seeding, so that even if
crystal growth
is slow you will see the streak line early on.
Enrico.
On Fri, 29 Apr 2011 12:43:21 +0200, anita p <crystals...@gmail.com> wrote:
Hi,
I had set up crystallization with a bicine as buffer and peg 400 as
precipitant. I used the detergent DDAO/LDAO as an additive to the
crystallization drop (one of the hampton additive screen condition, it
says
5% on the vial)
I have a clear drop and in the centre there is a shiny precipitate (looks
like granules attached to eah other).
Then I opened the drop to touch those granules with a needle, but
suprisingly its like a skin and the skin surrounded my needle, and then
some
how I was able to but back the skin in the ppt.
orelse the drop is clear if I remove the skin from the drop.
I couldnot see the same on the control drop with no protein and just
buffer.
Does anyone have any experience with such kind of of shiny skins on
crystallization drops??
Is it protein? Can I go forward and use it for seeding??
Please suggest
with regards
Rashmi
--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
http://www-dsv.cea.fr/en/institutes/institute-of-biology-and-technology-saclay-ibitec-s/unites-de-recherche/department-of-molecular-engineering-of-proteins-simopro/molecular-toxinology-and-biotechnology-laboratory-ltmb/crystallogenesis-e.-stura
http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
e-mail: est...@cea.fr Fax: 33 (0)1 69 08 90 71
