Dear all,
We have solved the sturcture of hen egg white lysozyme
with a barium ion at 2.7 fold data redundancy. Data collected at in-house
copper K-alpha source to 2.22 A resolution with 1 degree oscillation step
per frame. The substructure was correctly identified with just 8 frames ( up
to 7 frams the SHELXC did not produced the hkl files) when using SHELXD and
phasing was successful when using SHELXE-Beta trail version with 35 frames.
We can not belive it because the data used for substructure solution has
only 37% completeness. The crystallographers frequently says that in SAD
phasing to find the correct anomalous substructe the data reduendancy should
be high enough. But in our case the SHELXD was identified the heavy atom
site correctly at this very low completeness.
I welcome your suggestions and comments.
Thanks
with regards
Dhana
