[switch not too serious mode on]: well, it is lysozyme, which, according to diffraction properties, should perhaps be classified as a salt (LyCl7), not a protein... :-)
Mark J van Raaij Laboratorio M-4 Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC c/Darwin 3, Campus Cantoblanco E-28049 Madrid, Spain tel. (+34) 91 585 4616 http://www.cnb.csic.es/content/research/macromolecular/mvraaij On 4 May 2011, at 17:53, Dhanasekaran Varudharasu wrote: > Dear all, > > We have solved the sturcture of hen egg white lysozyme > with a barium ion at 2.7 fold data redundancy. Data collected at in-house > copper K-alpha source to 2.22 A resolution with 1 degree oscillation step per > frame. The substructure was correctly identified with just 8 frames ( up to 7 > frams the SHELXC did not produced the hkl files) when using SHELXD and > phasing was successful when using SHELXE-Beta trail version with 35 frames. > We can not belive it because the data used for substructure solution has only > 37% completeness. The crystallographers frequently says that in SAD phasing > to find the correct anomalous substructe the data reduendancy should be high > enough. But in our case the SHELXD was identified the heavy atom site > correctly at this very low completeness. > > I welcome your suggestions and comments. > > Thanks > with regards > Dhana
