Dear Rex,
for me it works in most cases. After all, vendors who sell inactive
proteins usually get troubles with their customers. However, as always
in crystallography, there is no guarantee that it will crystallize and
in this case you should contact the vendor to get a frozen solution. If
the protein you buy is not dirt-cheap (like trypsin), the price involved
is such that they will do it for you.
Best regards,
Herman
________________________________
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On
Behalf Of REX PALMER
Sent: Friday, May 06, 2011 10:01 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Lyophilized protein sample (purchased)
We have purchased a 5mg commercial sample of a protein we want to
crystallize.
On arrival it transpired that the sample had been lyophilized.
Does anyone know if this is likely to give problems and if so what
can/should be done about it?
Thanks in advance
Rex Palmer
Birkbeck College
