For some reason it would appear that the message below was not
distributed to ccp4bb when I sent it this morning, JISCMail
recommended that I resend the message, apologies if some have already
received it.
Andrew
Dear Munan,
First of all, you want to consider if the
apparent differences in cell dimensions of the two data sets are
actually significant or not. If you use the "Refine cell" option in
iMosflm, it will give an estimated error in the refined cell
dimensions. It is certainly unusual to get significantly different
cell parameters from the same crystal, unless there has been
significant radiation damage. Even then, the changes are usually very
small (much less than 1%).
If you want to process the two datasets with the same cell parameters,
this is very easy to do. If you are running iMosflm, you can simply
edit the cell parameters that appear on the images pane (click once to
highlight them, click again (over the numbers) to make them editable).
If you are running ipmosflm simply include a CELL keyword. There is no
need to reindex.
Best wishes,
Andrew Leslie
On 16 May 2011, at 22:40, Md. Munan Shaik wrote:
Dear all,
Can anybody give me some suggestion about data processing with
mosflm. I want to process one dataset with defining the cell
parameter.
I collect two dataset of the same crystal but when I am trying to
index and integrate then i saw some differences in the cell parameter.
I also tried to combine two MTZ with "sortmtz" but it fails. So,
now I tried to process second dataset with defining cell parameter
from the previous one. Please suggest me some ways.
thanks you in advance
regards
===================================================
Md. Munan Shaik
Department of Biotehnology
School of Bioscience and Biotechnology
via G. Colombo 03
Padova 35131, Italy
