But that's a lysine, so maybe a PTM ? Acetylated perhaps ? Jürgen
On May 18, 2011, at 1:56 PM, Shiva Bhowmik wrote: Hi Cedric, I presume you collected the datset at a synchrtron source. It could be free radical generated due to X-ray radiation has modified the glutamate residues. I have seen this happening for Ser residues. Cheers, Shiva On Wed, May 18, 2011 at 9:08 AM, cedric bauvois <[email protected]<mailto:[email protected]>> wrote: Hi, everyone, I am working on the refinement of a structure at about 1.4 Ang and found some clear extra density connected to a glutamate (see figure), but I don't know what it should be. The crystal came out from Tris-HCl buffer with peg600, and protein buffer contains sodium-phosphate. Does anyone figure out what this density can be ? Thanks for any help you can give. Cédric ...................... Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/
