Glutamate methyl ester? (or ethyl ester, which I'm not sure has ever been documented...hard to tell how many extra heavy atoms given the view direction in the image you supplied). Or the methyl/ethyl amides.
Dave From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Bosch, Juergen Sent: Wednesday, 18 May 2011 14:06 To: [email protected] Subject: Re: [ccp4bb] unknown density I take the lysine back, just realized wrong color coding it's an oxygen and not a nitrogen closer to the extra density :-) Jürgen On May 18, 2011, at 2:03 PM, Bosch, Juergen wrote: But that's a lysine, so maybe a PTM ? Acetylated perhaps ? Jürgen On May 18, 2011, at 1:56 PM, Shiva Bhowmik wrote: Hi Cedric, I presume you collected the datset at a synchrtron source. It could be free radical generated due to X-ray radiation has modified the glutamate residues. I have seen this happening for Ser residues. Cheers, Shiva On Wed, May 18, 2011 at 9:08 AM, cedric bauvois <[email protected]<mailto:[email protected]>> wrote: Hi, everyone, I am working on the refinement of a structure at about 1.4 Ang and found some clear extra density connected to a glutamate (see figure), but I don't know what it should be. The crystal came out from Tris-HCl buffer with peg600, and protein buffer contains sodium-phosphate. Does anyone figure out what this density can be ? Thanks for any help you can give. Cédric ...................... Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/ ...................... Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/
