I think you can get salt crystals from MgCitrate as well, so be forewarned!
Jacob On Mon, Jun 20, 2011 at 6:55 PM, Nat Echols <[email protected]> wrote: > On Mon, Jun 20, 2011 at 4:09 PM, Jayashankar <[email protected]> > wrote: >> >> Should I be happy if i get my crystals with one of the condition with a >> commercial kit >> containing citric acid and 40%MPD+ protein in(hepes+MgCl2)+ADP+VO3 > > Citric acid will chelate magnesium - this may not disrupt the > crystallization of the protein, but it could result in a non-physiological > active site. The coordination geometry of Mg2+ is hard to miss, so it's > usually obvious whether it's there or not despite being the same number of > electrons as water, but if you don't see a nice hexahedral arrangement, the > citrate took it all. > -Nat -- ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program cel: 773.608.9185 email: [email protected] *******************************************
