Dear McCully, I always used this protease with a cleavage success near to 100% at 4°C over night without shaking. Concerning the construct, I putted the first Met just after the sequence LEVLFQ/GP SSP, so I added only three aminoacids after the cleavage site.
All the best, Marco Il giorno 02/ago/2011, alle ore 17.52, Jerry McCully ha scritto: > Dear ALL; > > We are going to use the prescission protease cutting site > (LEVLFQ/GP ) in our cloning vector to remove the His6 tag. > > Do we need to insert some linkers between this cutting site and the > target protein to improve the cleavage efficiency? > > Or we are over concerned. > > Thanks a lot and have a nice summer. > > Jerry McCully > > > > > > > >
