Dear Jerry,
Our in-house series of vectors encode the Rhinovirus 3C-protease site, followed
directly by a NdeI site, which after cleavage leaves just GPHM on the front of
your protein. We routinely get 100% cleavage with incubation overnight at 4˚C
— and have several xtal structures to boot.
Best wishes,
Antony.
On 2 Aug 2011, at 17:03, Marco Lolicato wrote:
We are going to use the prescission protease cutting site (LEVLFQ/GP
) in our cloning vector to remove the His6 tag.
Do we need to insert some linkers between this cutting site and the
target protein to improve the cleavage efficiency?
Or we are over concerned.
Thanks a lot and have a nice summer.
Jerry McCully
