Dear All,
I am working on a structure using several sets of data collected between 5
to 6 angstroms. I have never worked with such low resolution before. I was
able to get pretty good TFZ between 10 and 16, and the electron density looks
overall pretty good. However, among the three data sets I have processed so
far, a large chunk of density is missing in all of them around the same area of
the protein (largely beta sheet). Is this a manifestation of some intrinsic
problems of the crystal, the data, or the protein? Is there a proper procedure
I can take to get this density back to make the map a bit more pleasing to view?
Thanks,
Huiming Li, Ph.D.
Immune Disease Institute
Children's Hospital Boston
