Hi, what is the completeness of the datasets across the whole resolution range (not overall - single number). At these resolutions missing a few reflections may be enough to hide quite a bit of structure.
Pavel. On Fri, Aug 12, 2011 at 10:32 AM, Huiming Li <[email protected]> wrote: > Dear All, > > I am working on a structure using several sets of data collected between > 5 to 6 angstroms. I have never worked with such low resolution before. I > was able to get pretty good TFZ between 10 and 16, and the electron density > looks overall pretty good. However, among the three data sets I have > processed so far, a large chunk of density is missing in all of them around > the same area of the protein (largely beta sheet). Is this a manifestation > of some intrinsic problems of the crystal, the data, or the protein? Is > there a proper procedure I can take to get this density back to make the map > a bit more pleasing to view? > > Thanks, > > Huiming Li, Ph.D. > Immune Disease Institute > Children's Hospital Boston > >
