I found this paper to be very helpful for a similar experiment. "Elucidation of the mechanism and end products of glutaraldehyde cross-linking reaction by x-ray structure analysis". Wine et al. Biotechnology & Engineering, Vol. 98, No. 3, October 15, 2007.
-----CCP4 bulletin board <[email protected]> wrote: ----- To: [email protected] From: Boaz Shaanan <[email protected]> Sent by: CCP4 bulletin board <[email protected]> Date: 08/15/2011 10:11AM Subject: Re: [ccp4bb] Protein crosslinking before crystallization Hi Opher, I seem to recall some old papers in which glutarldehyde was used (in the RESERVOIR only) in order to promote crystallization of proteins with suspected inter-domain flexibility. Unfortunately, I don't have specific references in mind. Cheers, Boaz Boaz Shaanan, Ph.D. Dept. of Life Sciences Ben-Gurion University of the Negev Beer-Sheva 84105 Israel E-mail: [email protected] Phone: 972-8-647-2220 Skype: boaz.shaanan Fax: 972-8-647-2992 or 972-8-646-1710 ________________________________________ From: CCP4 bulletin board [[email protected]] on behalf of Opher Gileadi [[email protected]] Sent: Monday, August 15, 2011 5:56 PM To: [email protected] Subject: [ccp4bb] Protein crosslinking before crystallization Hi all Does anyone have experience or insights on intramolecular crosslinking of proteins before crystallization? The idea is to lock a felxible (multidomain) protein into a restricted conformation. Thanks, Opher
