Hi Opher, I have a project where a protein was made 'cysless' and selective cysteines were introduced to allow for directed crosslinking using MTS and aldrithiol activated reagents (see Toronto Research Company and Pierce for a range of reagents of different lengths). A side product of these crosslinks was a dramatic improvement in crystal diffraction.
Katherine Rank Rayment Lab On 08/15/11, Opher Gileadi wrote: > Hi all > > Does anyone have experience or insights on intramolecular crosslinking of > proteins before crystallization? The idea is to lock a felxible > (multidomain) protein into a restricted conformation. > > Thanks, > Opher
