It possibly means: 1. Your sample application volume was too large 2. Total protein quantity was too large 3. Fraction size was too large 4. Sample was too crude (GEC is a finishing step, not a starting step for protein purification. 5. Sample was too concentrated/viscous 6. Flow rate was too fast 7. Ionic strength of buffer too low to suppress ion exchange effects.
Roger Rowlett On Aug 28, 2011 5:25 AM, "Allan Pang" <[email protected]> wrote: > Hi there everyone, > > What does it mean when you have proteins eluting in almost the whole > column volume of S200? > > I ran a gel with fractions from 8ml to 20ml and saw band for my > protein all throughout. > > Judging peaks on chromatogram is not useful as it doesn't have any > aromatic rings. > > Cheers, > > Allan > > -- > Allan Pang > > PhD Student > > G35 Joseph Priestley Building > Queen Mary University of London > London > E1 4NS > > Phone number: 02078828480
