Following on from Roger's fine suggestions: 8. Your column is knackered. Can you see fine lines or cracks in the column? Good packing is v.important for SEC columns.
HTH, Dave ============================ David C. Briggs PhD Father, Structural Biologist and Sceptic ============================ University of Manchester E-mail: david.c.bri...@manchester.ac.uk ============================ http://manchester.academia.edu/DavidBriggs (v.sensible) http://xtaldave.wordpress.com/ (sensible) http://xtaldave.posterous.com/ (less sensible) Twitter: @xtaldave Skype: DocDCB ============================ On 28 August 2011 10:25, Allan Pang <a.p...@qmul.ac.uk> wrote: > Hi there everyone, > > What does it mean when you have proteins eluting in almost the whole column > volume of S200? > > I ran a gel with fractions from 8ml to 20ml and saw band for my protein all > throughout. > > Judging peaks on chromatogram is not useful as it doesn't have any aromatic > rings. > > Cheers, > > Allan > > -- > Allan Pang > > PhD Student > > G35 Joseph Priestley Building > Queen Mary University of London > London > E1 4NS > > Phone number: 02078828480 >
