Hi Everyone,

Sorry for the naive and non-CCP4 question.

Is it possible to precipitate proteins (TCA, acetone) from a sample
that has already been stored in protein loading dye? The protein is
too dilute in my current sample and I basically want to load all of
the sample (100uL) in a single well in the gel. Unfortunately, I
already added protein dye with SDS and all.

Cheers and thanks.
Raji

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Raji Edayathumangalam
Instructor in Neurology, Harvard Medical School
Research Associate, Brigham and Women's Hospital
Visiting Research Scholar, Brandeis University

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