Is it ok for you to heat your sample? If so, you could "near-boil" your sample in a heating block, say at 95 degrees C, in order to "concentrate" it. I've done this in regular 1.5 mL eppendorf tubes with either the cap open or where I've punctured the top of the cap with a narrow bore needle (in order to relieve vapor pressure from water evaporation). At 95C, reducing 100 uL down to ~20 uL probably will take 15-30 minutes. If you're working on a membrane protein, this is probably not a route you can take.
Alternatively, maybe a speed vac will work? HTH- Brad On Fri, Sep 9, 2011 at 8:57 AM, Raji Edayathumangalam <[email protected]>wrote: > Hi Everyone, > > Sorry for the naive and non-CCP4 question. > > Is it possible to precipitate proteins (TCA, acetone) from a sample > that has already been stored in protein loading dye? The protein is > too dilute in my current sample and I basically want to load all of > the sample (100uL) in a single well in the gel. Unfortunately, I > already added protein dye with SDS and all. > > Cheers and thanks. > Raji > > -- > > ---------------------- > Raji Edayathumangalam > Instructor in Neurology, Harvard Medical School > Research Associate, Brigham and Women's Hospital > Visiting Research Scholar, Brandeis University >
