Dear Ritcha, You could also try low molecular weight PEG's, e.g. PEG400 in fairly high concentrations. They often do a good job in solubilizing ligands and are usually much more crystal-friendly than e.g. DMSO.
Best, Herman -----Original Message----- From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Chaudhary, Ritcha Sent: Friday, September 16, 2011 6:05 PM To: [email protected] Subject: [ccp4bb] Insoluble ligand? Hi All I have a ligand that is dissolved in 100% DMSO. The ligand crashes out even when diluted to 20% final DMSO concentration in the protein buffer. I have heard of people using detergents such as n octyl Beta D glucoside to keep the ligand solubilized at lower DMSO concentrations. Can someone give me pointers in this direction? Any suggestions, references etc..... Thanks much Ritcha
