Hi Christopher, you'll have to try to find out how they mix the components at MD and if they pH the solution afterwards or before (or not at all...) and to which pH. You can ask them; or try different mixing/pHing protocols on a small scale and see if one works in keeping all in solution. Mark
Mark J van Raaij Laboratorio M-4 Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC c/Darwin 3 E-28049 Madrid, Spain tel. (+34) 91 585 4616 http://www.cnb.csic.es/content/research/macromolecular/mvraaij On 27 Sep 2011, at 17:55, Browning Christopher wrote: > Dear All, > > My question might be a bit out of place, but perhaps someone can help. I've > screened my protein in the Nuc-Pro screen from Molecular Dimensions and found > some crystals in condition B10. They seem to be protein crystals as they are > not highly optically active and look different to salt crystals. So condition > B10 consist of 10% PEG 4K, 50mM Imidazole pH 7.2, 20mM Zinc sulfate. In the > screen, this condition is perfectly clear, but when I try and make my own > screen, the whole solution turns white. Apparently, Zinc sulfate/Imidazole > can be used for staining SDS-gels, but that does not really help my a lot. > Does anybody have an idea how I might be able to keep everything in solution, > seeing that the MD guys managed somehow...... I'm a bit desperate as I don't > have many hits for this protein. > > Cheers, > > Chris B > > -- > Dr. Christopher Browning > Post-Doctor to Prof. Petr Leiman > EPFL > BSP-416 > 1015 Lausanne > Tel: 0041 (0) 02 16 93 04 40
