Dear CCP4Pers, also j5 might be a good choice. I am not personally familiar with the software, so I would happy to hear any comments. It also seems to cope with modern cloning techniques like SLIC.
clicking away at http://j5.jbei.org/index.php/Main_Page Regards, Juha On 27 September 2011 18:42, Florian Schmitzberger < schmitzber...@crystal.harvard.edu> wrote: > Dear All, > > What type of software are people commonly using these days for > vector/plasmid map editing, making/visualizing vector maps, and aligning > (small to medium size) DNA sequencing data? Preferably, it should not be too > expensive and be able to write text files, readable by other programs. > > I am familiar with VectorNTI, which is great for vector visualization and > editing; but I find it somewhat expensive. Sequencher seems good to quickly > align DNA sequences (such as from DNA sequencing) with templates, but is not > free. I have been using ApE for while for alignments, but aligning many > sequences is more cumbersome than in Sequencher; I have not tested if > Sequencher is good at visualizing and editing plasmid maps. > > Ideally, I would like to have a single program for both purposes (vector > editing and DNA sequence comparison). Does something like that exist? What > are the alternatives to above programs? > > Thank you in advance. > > Florian > > ----------------------------------------------------------- > Florian Schmitzberger, PhD > Biological Chemistry and Molecular Pharmacology > Harvard Medical School > 250 Longwood Avenue, Seeley G. Mudd 123 > Boston, MA 02115, US > Tel: 001 617 432 5603 > > > > > > > > > > > > > > -- "Genius may have its limitations, but stupidity is not thus handicapped." -Elbert Hubbard ************************************************************************************ Juha Vahokoski Kalliotie 16 as. 10 90500 Oulu Finland mobile:+358 40 5286 778 ************************************************************************************