Re: [ccp4bb] help me after several refmac

[Eleanor Dodson]

On 10/13/2011 12:14 PM, 王瑞 wrote:
hello everyone:
     Excuse me, could anyone give me some suggestions? Afetr several cycles
of refmac, it give me such a result:
                                        Initial    Final
                     R factor    0.2540   0.2531
                        R free    0.3499   0.3500
      Rms BondLength    0.0151   0.0151
       Rms BondAngle    2.0266   2.0393
      Rms ChirVolume    0.1179   0.1188
  In fact, I have tried model building&  continue refmac, but the R free is
still above 0.34. The resolution is  43.728 to  2.088Å and space group is C
2 2 21 . What I should to do?

Thanks



Hard to say without seeing your map, model is probably correct.. Have 
you built waters? Are there errors? How good is your data?

 Eleanor