Sharp will do that. Or you can simply handpick them when looking at your anomalous map using the MR phases. With those sites you can use a variety of programs to refine the SeMet sites and hopefully get better phases.
Are you sure your MR model is correctly placed ? Have you tried conventional methods such as Shelx C/D/E to solve your SeMet SAD (?) data ? Jürgen On Nov 15, 2011, at 4:56 PM, Feng Guo wrote: Hi, there, Maybe someone asked this question before, but I couldn't find it in the archive. We use the native data to do molecular replacement before, but only part of the model fit the density. After collect a new set of selenoMet data, we try to use it to solve the phase, it solve some of the phase problem other than the MR, but still not complete. Is there anyway that I can somehow combine the two phases together? Thank you. Best, Feng ...................... Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://web.mac.com/bosch_lab/
