Hi Feng,
If your native and selenomet data are isomorphous, I would use your native
data, your selenomet data and your (partially correct) MR solution and
your heavy atoms altogether in refmac. At the moment, the refmac gui does
not support SIRAS refinement, but you can do it via script - Pavol Skubak,
Garib or I can show you.
Otherwise, you can try just the SAD selenomet data.
For SIRAS, check out this paper for more info:
Skubak et al (2009) Acta Cryst D, 1051-1061.
For SAD, check out
Skubak et al (2004) Acta Cryst D, 2196-2201
Skubak et al (2005) Acta Cryst D, 1626-1635.
A nice test case using the SAD function in refmac (at 3.8 Angstroms) on
RNA Polymerase II is described here:
Meyer et al (2009) JBC 284(19), 12933-12939.
Sorry for the obvious self-promotion, but I woke up in a Pavel Afonine-ish
type mood.
Best wishes,
Raj
On Tue, 15 Nov 2011, Feng Guo wrote:
Hi, there,
Maybe someone asked this question before, but I couldn't find it in the archive.
We use the native data to do molecular replacement before, but only part of the
model fit the density. After collect a new set of selenoMet data, we try to
use it to solve the phase, it solve some of the phase problem other than the
MR, but still not complete. Is there anyway that I can somehow combine the two
phases together? Thank you.
Best,
Feng
