Hello every one, Sorry for asking off topic question, I have purified different subunit of RNA polymerase, now i want to assemble it. Can you give me valuable suggestion regarding the gel filtration column and ion exchange column which will facilitate the purification of assembled RNA pol. I want to know name of gelfilteration column and other column which would be used to purify. I do not hav much knowledge regarding column so would you please help me by giving me your valuable suggestion.
Your suggestion would be highly appreciated. Thank you.