Which RNA polymerase are you working?

xiaopeng

----- 原始邮件 -----
发件人: "Appu kumar" <[email protected]>
收件人: [email protected]
发送时间: 星期五, 2011年 11 月 25日 上午 2:04:39
主题: [ccp4bb] RNA polymerase purification


Hello every one, 
Sorry for asking off topic question, I have purified different subunit of RNA 
polymerase, now i want to assemble it. Can you give me valuable suggestion 
regarding the gel filtration column and ion exchange column which will 
facilitate the purification of assembled RNA pol. I want to know name of 
gelfilteration column and other column which would be used to purify. I do not 
hav much knowledge regarding column so would you please help me by giving me 
your valuable suggestion. 

Your suggestion would be highly appreciated. 
Thank you. 

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