-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear Atul,
the "resolution" of your data set would affect the whole unit cell and not just the region of 30-40 residues. The more likely reason is a disordered N-terminus. There is nothing to do about this in silico. It may be worth recloning your protein with that N-terminal part chopped off - there is a fair chance that the product is more stable and hence diffracts much better than 3.2A. Regards, Tim On 12/08/2011 07:47 AM, atul kumar wrote: > Dear all > > I have crytals which diffract up to 3.2 A at synchrotron, I am solving this > by molecular replacement. I have built 70% of the model successfully,but > the problem is it have a very poor density for some 30-40 residues at N > terminal. I can't build anything in this region,this could be because of > disordered structure or because of low resolution.what are the things which > I can try to improve this? > > suggestion are requested! > > thanks > regards' > Atul Kumar > - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.10 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFO4G0GUxlJ7aRr7hoRAvaIAJ9d+zQW8BXFYDyfE75Kl/qyQnTB8wCg29ce /ALmC4aGLkatNQgaCzvrlAA= =bUCq -----END PGP SIGNATURE-----
