Theresa You should also try microseeding into *random screens *by making a seed stock with the crystals that you have, to use with both microbatch and vapor diffusion experiments. You will often pick up new and better conditions and you're more likely to get well-formed crystals right out of the screens.
I hope it works Patrick _________________ Refs: Allan D’Arcy, Frederic Villarda, May Marsh. An automated microseed matrix-screening method for protein crystallization. Acta Crystallographica section D 63 (2007), 550–554. Galina Obmolova,* Thomas J. Malia, Alexey Teplyakov, Raymond Sweet and Gary L. Gilliland. 'Promoting crystallization of antibody–antigen complexes via microseed matrix screening.' Acta Crystallographica Section D 66 (2010) 927–933. Open-access at http://journals.iucr.org/d/issues/2010/08/00/bw5361/bw5361.pdf A. G. Villaseñor, A. Wong, A. Shao, A. Garg, A. Kuglstatter and S. F. Harris. 'Acoustic matrix microseeding: improving protein crystal growth with minimal chemical bias.' Acta Crystallographica Section D 66 (2010) 568-576. Gregory Ireton and Barry Stoddard. 'Microseed matrix screening to improve crystals of yeast cytosine deaminase'. Acta Crystallographica section D60 (2004) 601–605. Patrick D. Shaw Stewart, Stefan A. Kolek, Richard A. Briggs, Naomi E. Chayen and Peter F.M. Baldock. 'Random Microseeding: A Theoretical and Practical Exploration of Seed Stability and Seeding Techniques for Successful Protein Crystallization'. Cryst. Growth Des., 2011, 11 (8), pp 3432–3441. On-line at http://pubs.acs.org/doi/abs/10.1021/cg2001442. * If you don't have a subscription to Crystal Growth and Design, click **here*<http://www.douglas.co.uk/mms.htm#CGDFreeCopy> * to obtain a free copy (we're limited to 50 downloads in the first year).* On 26 January 2012 15:33, Theresa H. Hsu <theresah...@live.com> wrote: > Dear crystallographers > > I have a protein of 90 kDa forming dimers. Crystals formed with microbatch > and vapor diffusion method in 24 hours but no diffraction at home source. > Dissolved crystals was confirmed to be the protein with mass spec. > > Any suggestions to improve diffraction would be welcome. > > Thanking you in advance. > > Theresa > -- patr...@douglas.co.uk Douglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36
