This might be obvious, but make sure you washed the crystals thoroughly before dissolving them for SDS-PAGE or mass spec
-Jesse On Thu, Jan 26, 2012 at 10:48 AM, Katherine Sippel <[email protected]> wrote: > Might I suggest consulting the CCP4 user community wiki on the topic: > > http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Improving_crystal_quality > > Good luck, > > Katherine > > > > On Thu, Jan 26, 2012 at 9:33 AM, Theresa H. Hsu <[email protected]> > wrote: >> >> Dear crystallographers >> >> I have a protein of 90 kDa forming dimers. Crystals formed with microbatch >> and vapor diffusion method in 24 hours but no diffraction at home source. >> Dissolved crystals was confirmed to be the protein with mass spec. >> >> Any suggestions to improve diffraction would be welcome. >> >> Thanking you in advance. >> >> Theresa > >
