Dear Dianfan, In some cases the ATP-lid of the kinase is blocking the active site in the crystal form. In those cases the only option is to try co-crystallisation.
Besides ATP and the homologs you mention you can also try ADP that as you will see in the PDB has been heavily used for kinases. Best of luck Sofia Sent via BlackBerry® from Vodafone -----Original Message----- From: Dianfan Li <[email protected]> Sender: CCP4 bulletin board <[email protected]> Date: Wed, 1 Feb 2012 19:17:03 To: <[email protected]> Reply-To: Dianfan Li <[email protected]> Subject: [ccp4bb] Soaking Kinase Crystals with ATP analogues Dear all, Sorry about a non-crystallographic question here. I am working on a kinase and would like to get an ATP analogue into the crystals. When soaked with AMP-PCP, the kinase crystals crack in about 15 min at 4 C. I could try other analogues like AMP-PNP etc, but those would probably behavour in a same way as AMP-PCP. Is it a good idea of trying quick soaks at high concentrations of AMP-PCP? Co-crystallization is another option I have but AMP-PCP is a substrate of the kinase (with low rate). What are other ways of getting ATP analogues into a crystal? Thanks for suggestions, Dianfan Dianfan Li, PhD College of Biochemistry and Immunology Trinity College Dublin Dublin, Ireland.
