Consider cross-linking crystals with glutaraldehyde. The caveat here is
that you may end up with the protein conformation that is forced by
lattice, but if the issue is just the fragility, you should be fine. I
assume that crystals simply crack but do not dissolve?
Certainly, as others have said, slower inhibitor addition should be
tried.
On Wed, 2012-02-01 at 19:17 +0000, Dianfan Li wrote:
> Dear all,
>
> Sorry about a non-crystallographic question here.
>
> I am working on a kinase and would like to get an ATP analogue into
> the crystals. When soaked with AMP-PCP, the kinase crystals crack in
> about 15 min at 4 C.
>
> I could try other analogues like AMP-PNP etc, but those would probably
> behavour in a same way as AMP-PCP. Is it a good idea of trying quick
> soaks at high concentrations of AMP-PCP? Co-crystallization is another
> option I have but AMP-PCP is a substrate of the kinase (with low
> rate).
>
> What are other ways of getting ATP analogues into a crystal?
>
> Thanks for suggestions,
>
> Dianfan
>
> Dianfan Li, PhD
> College of Biochemistry and Immunology
> Trinity College Dublin
> Dublin, Ireland.
--
"Hurry up before we all come back to our senses!"
Julian, King of Lemurs
