if you use oil do direct dry Paratone-N, with paraffin oil is not as good. Li-salts should work also - i would almos imgaine you can freeze directly from so high (NH4)2SO4 conc. but perhaps not. little bit (10%) glycerol probably does it also..
Tommi On Feb 6, 2012, at 12:55 AM, Vineet Gaur wrote: > Hi Theresa, > Once I had crystals in 3.5 M Amm. Sulfate. I used Paraffin oil and > Paraton-N-oil (in 1:1 ratio). I also used 30% Xylatol. > > Best, > Vineet > > On Sun, Feb 5, 2012 at 5:49 PM, Theresa H. Hsu <[email protected]> wrote: > Hi all > > Is there a list of conditions to be tried *first* for cryoprotectant? My > crystals diffract at room temperature capillary but no in 30% PEG 400. > Crystals are from 2 M ammonium sulfate. > > Thank you. > > Theresa >
