Hello Jiahong: If I understand correctly that you want to test protein-protein interaction or inhibition study in solution, maybe you can try something like ELISA to test protein-protein interaction. Or if your B protein has 6 histag, you can use Ni-NTA agrose beads to test inhibition or binding depending on your purpose. And another option (a little "dangerous" ), is using radio active to label one of your protein.
Yu Xiaodi Date: Wed, 8 Feb 2012 14:17:47 +0000 From: [email protected] Subject: Re: [ccp4bb] Dye for protein affinity measurement To: [email protected] Jiahong Thermo sells a series of kits called DyLight Fluor for fluorescent labelling of antibodies or other proteins. They have everything you need and they're very convenient and easy to use. You can pick the excitation and emission wavelength. If you label both A and B (or C) with different "colors" you will be able to see if both are in your crystals (assuming crystallization is part of your approach). You need only label a small percentage of your protein or peptide to see whether the protein is present in a crystal. Patrick http://en.wikipedia.org/wiki/DyLight_Fluor Forsythe, E.L., Achari, A., and Pusey, Marc L. (2006), Trace Fluorescent Labeling for High Throughput Crystallography, Acta Cryst. D62, 339-346. We used DyLight 350 NHS Ester to check we had protein crystals - see methods section of Cryst. Growth Des., 2011, 11 (8), pp 3432–3441 2012/2/8 Jiang Jiahong <[email protected]> Dear all, I am looking for some kind of dye for protein affinity comparison, but do not know which to choose. I know protein A can contact B to form a complex,now I hope to find something simiar with A to act as an inhibitor to block the process of A-B complex formation. Maybe a short peptide, a segment of protein A or even some organic molecule. Because here is a poor access to ITC nor Biacore, I can only rely on some dye to check the competence between A and inhibitor candidates. If any one can offer any suggestions. That would be so grateful! Any way,thank kind-hearted people in advance! Regards Jiahong -- [email protected] Douglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36
