Giorgo, We have done that routinely for quite some time now. We had problems when using a normal CCD detector, where we had to collect two or three sweeps to avoid overloads (see below). Since we have the PILATUS this is not necessary anymore and the data behaves fine. "Problems" still persisting are: we have only a single axis goniometer, which can lead to low completeness in P1 and P-1. Highest energy (17keV) and closest distance (188mm) at our beamline have many SM crystals (even the ones that "don't diffract" in house -- that is a 300 or 500 u sealed tube) with an I/sig of 5-10 at the edge of the detector. Crunch, Acorn, ShelxCDE and ShelxS don't have any problem with any of the data we collected to <0.9A resolution. The multipass caused some inexplicable non definite positives during refinement. We haven't tracked that down systematically, so it might just have happened haphazardly.
HTH, Jens On Wed, 2012-02-08 at 11:41 +0000, Giorgio Giardina wrote: > Hello, > I have some interesting small molecule xtals. > I was wondering if it is possible to collect a small molecule data-set using > a sincrotron macromolecular xtallography beam line, maybe with a very low > beam intensity and moving the detector as close as possible? > Has anybody experienced that? > And if I get the images back home, can I process them using standard > macromolecular software or do I need ab-initio special programs? > Will MR work for phasing? > > Thanks in advance, > Giorgio
