Actually, I would refer the ccp4-bbs to Journal of Structural Biology 175 (2011) pp216-223 for the use of fluorescence in relation to protein crystallization.
Regards, Bryan -------------------------------------------------------------------------- Confidentiality Notice: This message is private and may contain confidential and proprietary information. If you have received this message in error, please notify us and remove it from your system and note that you must not copy, distribute or take any action in reliance on it. Any unauthorized use or disclosure of the contents of this message is not permitted and may be unlawful. -----Original Message----- From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Christian Roth Sent: Tuesday, February 28, 2012 8:09 AM To: [email protected] Subject: Re: [ccp4bb] Desalting columns If you want test a lot of different conditions a Thermofluorecence assay might work for your protein and you may find a condition which stabilise your protein. However there is no warranty that it crystallise better afterwards. Christian Am Montag 27 Februar 2012 17:01:33 schrieb Sangeetha Vedula: > Dear bb users, > > I am trying to crystallize a ~320 kDa protein that crashes out if > concentrated past about 3 mg/mL. > > I would like to try to exchange it into various buffer-salt-additive > combinations to see which buffer works. For a starting point, I'd like to > use desalting colums. > > Does anyone have suggestions for good buffer exchange and sample recovery? > I woud like to load about 250 uL onto each column. > > Thanks a lot! > > Best regards, > > Sangeetha. >
