I'd suggest: - Find a dinosaur from my generation who can suck one into a capillary and check diffraction at room T.
- Try using those loops that look like miniature tennis paddles to give the crystal a little more support - To minimize strain on the crystal when pulling it out of the drop, try to get its long dimension perpendicular to the air-water interface (usually easier said than done). - Try to find conditions where the crystals don't start to redissolve while you mount them ---- Original message ---- >Date: Fri, 13 Apr 2012 18:51:58 +0800 >From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> (on behalf of Prem kumar ><pk_ai...@yahoo.com>) >Subject: [ccp4bb] Crystal behave funny >To: CCP4BB@JISCMAIL.AC.UK > > Hi all, > I got some Protein + DNA complex crystals (image > attached) recently. > They are needle shape some times splitted chromosome > type crystals. When we pick long needles they bend > so much than normal crystal but they dont break. The > small needle dissolve very fast as try to open the > drop's film. we try to diffract the long needle > crystals and they diffract up to 20 A resolution. > Any suggestion how to improve those crystal packing. > Thanks in advance! > -Prem >________________ >IMG_1438.JPG (2343k bytes)
